Whole-mouse imaging the hard way: Clearing, then slicing + block-face imaging with beam-scan / sensor scan-line synchronization and continuous stage movement in an oblique imaging setup (ViSOR).
This time, they scaled it up to whole-mouse and used pre-cleared mouse bodies (ARCHmap-blockface-ViSOR):
High-speed mapping of whole-mouse peripheral nerves at subcellular resolution
Shi et al., preprint at biorxiv 2025
https://doi.org/10.1101/2025.01.22.632569
Here's a #MicroscopyMonday segmentation of some neurons from the optic lobe, or visual processing area of the #Drosophila fruit fly's brain. The #connectome for the full optic lobe was released in 2024 by #HHMIJanelia, Google, and the University of Cambridge, with support from the Wellcome Trust.
This segmentation of #MicroscopyMonday data reconstructs neurons of the "MANC", or male adult nerve cord, from the #Drosophila fruit fly. The nerve cord is like a spinal cord and connects the brain and muscles. #HHMIJanelia, Google, and the University of Cambridge released this work in 2023.
A use of #MicroscopyMonday data is making segmentations. Here's one, of #Drosophila protocerebral bridge and nodulus neurons, from the 2020 Hemibrain by #HHMIJanelia and Google. Michał Januszewski and Viren Jain's team made an initial segmentation with #AI flood-fill algorithms...
Ya boi is back with more microscope photos :3
Here are a few pics of a cool looking filamentous algae I found looking at more bog water under the microscope. I don't know too much about algae species (yet!) so I can't say much in terms of identification for this specimen, but it's certainly pretty to look at!
#microscopy #microscopymonday #algae (does this really count as #NaturePhotography? 
)
#MicroscopyMonday – No this is NOT an aerial view of the Grand Canyon.
This is a scanning electron microscopy image of the cranial sutures in the rat skull. 
These are fibrous joints where the bones of the skull interdigitate, allowing for flexibility and growth during infancy and early childhood before fusing in adulthood.
Happy #MicroscopyMonday!
Here is a kymograph of a cell crawling into a microchannel. Each horizontal row of pixels represents a line drawn from outside (green) through a channel (purple) at 5 second timepoints. ~1/2 way down (20 min) a cell fully enters the channel and takes off!
Happy #MicroscopyMonday! Here are somme amoebae crawling into channels
LUT: JDM_Phase Ink Mint-Cherry
Pre-vacation #MicroscopyMonday magic rendered by @Daniel_Stabley! This is a showstopper: our top plasma membrane probes light up neurons and glia. But wait, there's more! The grand finale? A lattice light sheet stunner showing off its isotropic prowess in the XZ view. Confocal 3D rotations, eat your heart out! 
#Neuroscience #MicroscopyMagic #NextLevelImaging #cellbio #cellfie #scicomm #neuroscience #Microscopy
That's what a leaky blood vessel looks like in the microscope. Astrocytes (
Gfap) around the blood vessel become reactive, inducing neuroinflammatory responses and attacking the surrounding neurons (
Neun) in the pathway. #MicroscopyMonday
#LongCovid
Happy #MicroscopyMonday, from an amoeba crawling in a very narrow channel!
Ich habe ein bisschen USB 3.0 Kabel zerschnitten und Querschnittsbilder mit dem Mikroskop gemacht. Das Video hat Kapitelmarken für die Eiligen und noch mehr Infos und Links in der Videobeschreibung. Viel Freude!
#Mikroskop #MicroscopyMonday #dieKleinenDinge
https://youtu.be/iIYT0736AQQ?feature=shared
Es ist zwar nicht Montag, aber ich hab lange nichts mehr zu #MicroscopyMonday gepostet, also:
Querschnitt durch ein USB3 Kabel, hier zu sehen einer der zwei Zusätzlichen DatenKanäle im USB3 Standard (lila & Orange) mit unisolierter zusätzlicher Schirmung. #Miroskop #Mikroskopie #dieKleinenDinge
Bild ist CC BY 4.0
Let's have a #notthecover for #MicroscopyMonday: hippocampal neuron with endogenously tagged HA-actin — one of the proposed covers for https://rupress.org/jcb/article-abstract/222/10/e202208110/276185/Presynapses-contain-distinct-actin
Es ist Montag, also auch #MicroscopyMonday
Im Video seht ihr eine Mikroskopie-Aufnahme von einem Mehrklingen-Rasierer, den ich langsam kippe, so dass man die Schneide-Kante einer der Klingen gut erkennen kann. Das Video stammt aus einem ganzen Mikroskopie-Stream über Messer und Klingen, hier findet ihr einen Zusammenschnitt davon: https://www.youtube.com/watch?v=9LRrt-hsuYk
Heute ist Montag, also auch #MicroscopyMonday
Ich habe mir mal Stifte und die entsprechenden Linien auf Papier unter dem #Mikroskop angeschaut, und das ganze sogar live gestreamt. Auf den Bildern unten seht ihr die Striche auf Papier, eine Kugelschreiber-"Spitze" und eine Nahaufnahme eines Farbdrucks. Maßstab jeweils im Bild. Bilder CC BY 4.0
Zusammenschnitt des Streams: https://youtu.be/sXN1yjkmCik?feature=shared
Heute ist Montag, also auch #MicroscopyMonday
Ich habe mir mal die grauen Reflektorstreifen einer Warnweste unter dem Mikroskop angesehen (war in einem früheren Mikroskop-Stream auf #twitchDE) - diese Reflektorstreifen bestehen aus kleinen Glaskügelchen mit einem Durchmesser von ca. 40-70µm, eingebettet in eine Art Gel.
Bilder sind CC BY 4.0
Happy (or not?) #MicroscopyMonday!
I made a chart out of all the amoeba faces I've found to put on the lab fridge. Unfortunately, happy faces are not an observable phenotype for Naegleria.
Better late than never for #MicroscopyMonday!
Here is a z-stack (every focal plane moving vertically through a sample) of actin polymer-stained Naegleria amoebae, followed by a maximum intensity projection (the brightest xy point of all the z positions). The darker colors here represent places where there is more actin polymer.
